Current grant projects

FNO / SGS Grants

Separation algoritm for fluorescent-based sorting of cells from monoclonal gammapaties

Optimalisation of collection and preprocessing of patient bone marrow and periferal blood samples for cytogenetic, genomic and proteomic analyses.  Setting up the flow cytometry and flourescent activated cell sorting (FACS) and finding proper algoritms for sorting cells from monoclonal gammapties (plasma cells).  The pure populations of plasma cells will be used for isolation of DNA/RNA and proteins.  (2015-2018)

Whole genome screening in Waldenstrom macroglobulinemia

Optimalisation of FACS and MACS-based isolation of WM cells.  Standardisation of the AS-PCR method for rutine examination of MYD88 and CXCR4 genes from patient bone marrow and periferal blood samples. A whole genome amplification and comparative genomic hybridisation (aCGH). Determination of predictive values of bone marrow vs periferal blood samples.

Unique serum miRNA profile in patients with imunoglobulin light chain amyloidosis

Comparism of expression profile of serum miRNA in imunoglobulin light chain amyloidosis (ALA), monoclonal gammapaty of undertermined significance (MGUS) and multiple myelom (MM).  Identification and validation of unique miRNA profile in ALA samples using quantitative real-time PCR. Correlation of miRNA pattern with other clinically relevant parameters.

Analysis of mutation profile of residual aberant plasma cell clones: towards a new level of personalised medicine for monoclonal gammapaties

Determination of a minimal amount of cells from monoclonal gammapties required for reliable whole genome amplification (WGA). Validation of WGA using multiple genomic approaches: PCR, aCGH and NGS. This project is a part of a larger study, where the long term goal is the identification of the crucial mutations in the funding clones of the monoclonal gammapaty of undertermined significance (MGUS) and in the resistant, residual clones from multiple myeloma (minimal residual disease, MRD).

In vitro differenciation of human plasma cells

Establishing a novel protocol for the in vitro differenciation of PC from human haematopoietic progenitor using a specific set of cytokines. The cells will serve as a model system for further genetic experiments (CRISPR, etc.) to study development of multiple myeloma, iteraction with other cell types (microenviroment) and resistance to specific drugs.

 

Regional grant from the Moravian-Silesian Council

Strengthening international cooperation in science, research and education

Project ID: 01211/2016/RRC
In this project we aim to atract talended and highly motivated scientists from abroad to set up new methods and projects in our laboratory. Currently, we are pleased to host Dr. Michal Simicek, who joined us in 2017 after completing his postdoctoral reaserch in the Medical Research Council in Cambridge. Dr. Simicek will be responsible for setting up mammalian cell culture laboratory to study tumor microenviroment and  its contribution to drug resistance in multiple myeloma.

 

Czech Health Research Council Grants

In-depth genomic analysis of residual clone in multiple myeloma: approach for individualized targeted therapy

Project ID:  17-30089A
Whole genome sequencing using next generation sequencing technology and gene expression profiling of multiple myeloma (MM) cells isolated from patients with the minimal residual disease to identify mutations and expression patterns typical for resistant and/or refractory MM disease.

Aberrant plasma cells clonal diversification in immunoglobulin light chain amyloidosis

Project ID:  16-06666S
Dissection of a complex genetic processes in the evolution of clonal, aberrant plasma cells in patients with monoclonal gammapaties. Genomic DNA from patients with multiple myeloma, amyloidosis and both togehter is amplified and examined by the next generation sequencing. Further bioinformatic analysis is performed to disentangle separate evolutionary lineages.

 

Czech Ministry of Education, Youth and Sports: Institutional developmental project grants

Complex genomic studies in monoclonal gammapaties